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Our laboratory has started from 2010 within a framework of the Plant Science Global Top Education Program, supported by Ministry of Education, Culture, Sports, Science and Technology. Aim of this program is to establish an educational system linking nationwide universities and institutes, and cultivate young plant researchers for promotion of future plant science in Japan.
With the goal to promote education on the frontier technologies, we focus rapidly advancing research fields such as genome-transcriptome analysis, biochemical and proteomic analysis of intracellular protein complexes, and visualization of those protein-protein interactions in living cells. Four researchers are assigned for this education purpose; Dr. Tetsuya Kurata has a expertis for genome-transcriptome analysis with high-throughput next generation sequencer; Dr. Masayuki Fujiwara is a biochemist whose focus is on the protein purification with various biochemical methods; Dr. Yoichiro Fukao specializes in the proteomic analysis using latest mass spectrometers; Dr. Noriko Inada is an expert of bioimaging using confocal and multiphoton microscopies.
Every year, we recruit ~30 graduate students among those who applied their own research proposals employing genome-transcriptome analysis, biochemical and proteomic analysis, or bioimaging analysis investigating plant protein function. Recruited graduate students will benefit from one-week training of above-mentioned cutting-edge technologies and avid interaction with other graduate students participating from various universities and institutes.
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- Genome-Transcriptome analysis (Tetsuya Kurata)
- Multi-omics study for coordinated growth control in Arabidopsis.
- Development of analytical system for genome, epigenome and transcriptome from small amount of plant sample.
- Purification and Analysis of Protein complex (Masayuki Fujiwara)
- Study for protein complex regulating plant innate immunity.
- Analysis of CDD complex regulating the photomorphogenesis in plants and the interacting proteins
- Development of analytical method for protein complex and protein-protein interaction.
- Plant Proteomics (Yoichiro Fukao)
- Proteomics of mineral-stress responsive proteins in Arabidopsis.
- Establishment of absolute quantification method for protein phosphorylation by MS-based proteomics.
- Bioimaging analyses (Noriko Inada)
- Temperature imaging of living cells using Fluorescence Lifetime Imaging Microscopy (FLIM)
- Imaging powdery mildew-Arabidopsis interaction
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- Fukao Y. et al., Plant Physiol., in press, 2011
- Fukao Y. et al., Plant Cell Physiol., 50, 2234-2239, 2009
- Uchida N. et al., Plant Cell Physiol, in press, 2011
- Ishida T. et al., Annu. Rev. Plant Biol. 59, 365-386, 2008
- Chandran, D., Inada, N., et al., PNAS 107, 460-465, 2010
- Iwai, M., Yokono, M., Inada, N., and Minagawa, J. PNAS 107, 2337-2342, 2010
- Chen L. et al., Cell Host Microbe. 7, 185-96, 2010
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Fig. 1 Coordinated growth among cell layers in Arabidopsis hypocotyl, and ChIP-seq data from NGS.
Fig. 2 LTQ-Orbitrap XL and TSQ-Vantage.
Fig. 3 Imaging powdery mildew-Arabidopsis interaction
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